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Investigation of enzymes and isoenzymes

Isozyme 1 lactate dehydrogenase in the blood

Isoferments of lactate dehydrogenase are contained in tissues in a strictly defined ratio, that is, each tissue, including blood, has a characteristic spectrum of lactate dehydrogenase isoenzymes.

Troponin I in the blood

Troponin I is a structural protein of the troponin complex of muscles with a molecular weight of 26,500 Da. Troponins I, like troponins T, cardiac and skeletal muscles, differ significantly in their amino acid sequence.

Troponin T in the blood

The troponin complex is part of the contractile system of muscles. It is formed by three proteins: troponin T, which forms a link to tropomyosin (molecular weight 3700), troponin I (molecular weight 26,500), which can inhibit ATPase activity, and troponin C (molecular weight 18,000), which has a significant affinity for Ca2 + .

Myoglobin in the blood

Myoglobin is a gem-containing chromoprotein; is a light chain of myosin with a molecular weight of 17.6 kDa. It is a protein transporting oxygen in skeletal muscles and myocardium.

MB-fraction of creatine kinase in serum

Creatine kinase in the cardiac muscle consists of two isoenzymes: KK-MM (60% of total activity) and KK-MB (40% of total activity). KK-MB - dimer, consists of two subunits: M (muscle) and B (cerebral).

Total creatine kinase in the blood

Creatine kinase reversibly catalyzes the phosphorylation of creatine. The most rich in creatine kinase is skeletal musculature, the heart muscle, less in the brain, thyroid gland, uterus, lungs.

Markers of myocardial damage

Myocardial infarction is an acute disease that arises from a sharp discrepancy between myocardial oxygen demand and its delivery through the coronary arteries, which results in the development of necrosis of a part of the heart muscle.

Angiotensin-converting enzyme (apf) in the blood

Angiotensin converting enzyme (ACE) is a glycoprotein, which is present mainly in the lungs and in small amounts in the brushing of the epithelium of the proximal tubules of the kidneys, the endothelium of the blood vessels and the blood plasma.

Acidic phosphatase in the blood

Acid phosphatase in the blood should not exceed 5-6.5 IU / l, and ideally it should not be in the bloodstream at all. In general, phosphatases - a special kind of enzymes, which are considered "water" - hydrolases. These substances can be found not only in the human body, but also in tissues, in the organs of almost all animals, and even in all kinds of plants.

Pancreatic elastase-1 in feces

Human pancreatic elastase-1 belongs to the family of acidic elastases. It is present in the secretion of the pancreas and feces. The enzyme does not break down when passing through the intestine.

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