After delivery to the laboratory of the material, a study is being conducted. The biological material is transferred to a centrifuge, clotting factors are removed, thus obtaining pure serum. Then, further study of the obtained serum is carried out. The study can be conducted by different methods. Most often use liquid chromatography or immunochemiluminescent analysis. Most often, the second option is used, because it is more convenient to use, it is much easier and faster. From the economic point of view, it is also more profitable.
The principle of the method consists in binding 25-hydroxycalciferol to paramagnetic particles that are coated with antibodies. After this, the particles precipitate with a magnet, and they are washed. A suspension is formed in which polyclonal antibodies and various reagents are added. As a result, complexes are formed that have luminescent properties. With the help of special equipment it is possible to estimate the intensity of luminescence. Based on these data, the concentration of vitamin D is calculated.
Blood test for vitamin D
The procedure begins with blood sampling from the vein. With all the rules of asepsis, venipuncture is performed, the necessary amount of blood is taken. The technique may vary somewhat, depending on the method used. In most cases, the blood is initially taken into a tube containing EDTA. This gel, which prevents blood clotting and changing its properties. After the procedure is completed, the puncture site is pressed down with a cotton ball, the arm is bent at the elbow and asked to hold in this position until the bleeding stops completely.
Blood can be stored for some time in a special box, in a sealed tube. In this form, it is usually transported to the laboratory. Frozen blood is not produced.
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Urine analysis for vitamin D
The vitamin D content is often examined by urine analysis. A Sulkovich sample is used for the determination. It makes it possible to reveal the calcium content in the blood, on the basis of which they draw further conclusions about the content of vitamin D.
The results of the analysis are qualitative, defined as follows: "-" indicates a deficiency of vitamin D, "+" or "++" indicates a norm, "+++" indicates excessive content.
Analysis for calcium and vitamin D
It allows to determine the presence of calcium in the urine. According to these data, it is possible to judge the peculiarities of the metabolism of vitamin D. The study was called Sulkovich's trial, or completely urine analysis by the Sulkovich method. Gives information only about whether there is calcium in the urine. In what quantity it is allocated, it is impossible to determine.
Especially important is the analysis for children, because they have a high risk of abnormal development, the occurrence of rickets with a lack of vitamin D and a violation of calcium metabolism. If the child was born in winter or autumn, this risk increases because of the lack of sunlight. If a deficiency is found, the vitamin should be added to the body artificially, and it is important to control the dosage. The drawback entails breaking the bones, reducing strength. Most often these are the first signs of rickets. Subsequently, rickets are manifested in the form of curved legs, a disproportionately large head, a forward belly. Measures should be taken urgently, otherwise the ossification of the skeleton will occur and the person will remain with such pathologies for life.
Excess calcium is also dangerous. Develops convulsive syndrome, aesthetic appearance is disrupted. Excess calcium and vitamin are deposited in the bones, as a result of which their growth slows down significantly.
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Analysis for vitamin D 3
The most accurate content of vitamin D can be determined by the amount of vitamin D 3 (25-hydroxyvitamin D 3 ) . Usually, a comprehensive study is conducted, during which this parameter is determined. The fact is that the complex analysis of "25 OH D3" implies the study of the two components of this vitamin - D2 and D3. The source of vitamin D2 is food, while ultraviolet rays also serve as a source of vitamin D3. The study is subject to serum.
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Analysis for the assimilation of vitamin D
The most accurate picture describing the nature of the absorption of vitamin D in the body can be obtained by conducting a comprehensive blood test. Children have enough urine analysis.
Analysis for vitamin D to the baby
For children with a view to determining vitamin D, urine tests are performed. The method is simple, does not require any preliminary preparation. It is only necessary to collect the morning urine and carry it to the study. Urine is collected in the morning, immediately after the baby woke up after a long sleep. This is done before the child begins to drink or eat. For young children use urinals, acting as a reservoir for urine. Urine dumps are disposable.
The technique of the study is also not difficult. In the laboratory, the urine is mixed with a Sulkovich reagent, which is a mixture of calcium salts and oxalic acid. As a result, there is turbidity, the degree of severity of which is judged about the presence or absence of calcium in the urine. If this result does not fully clarify the clinical picture of the pathology, or it is not enough to diagnose, predict, investigate daily urine.
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Analysis for vitamin D adults
Adults determine the vitamin content in venous blood. With the help of simple laboratory technology, the concentration of vitamin in the serum is detected. In adults, impaired calcium metabolism is fraught with numerous serious disorders. The main violation is osteoporosis. In the body of an adult vitamin D takes part not only in the exchange of calcium, but also in the exchange of phosphorus. As a result of deficiency, muscle weakness, tremor develops. Violated the work of all internal organs, including kidneys, muscles. Violated regulation of blood pressure, normal activity of the heart.
A normal amount of vitamin D is necessary for the proper functioning of the thyroid gland. The immune system rises, blood coagulability normalizes. The exchange of other vitamins and microelements also depends on the normal content of vitamin D. With a low or excessive vitamin content, the work of the kidneys and liver can be disrupted.
One of the important properties is the provision of normal cell multiplication, in which their uncontrolled division does not occur. Blocking uncontrolled reproduction of cells is the key to successful treatment and prevention of cancer. Also, the activity of the immune system increases, resulting in the suppression of autoimmune diseases. This property provides prolongation of life in immunodeficiencies and AIDS.
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Analysis of vitamin D in pregnancy planning
The analysis is mandatory for pregnant women, it is planned. This is due to the fact that this vitamin is very important for both the mother and the future child. Promotes the normal exchange of calcium and phosphorus, regulates the multiplication and division of cells, their differentiation. Takes part in the synthesis of hormones. Forms in the main degree the skeleton of the unborn child. Prevents the development of varicose veins in the mother, preserves the beauty and health of hair, nails, teeth. If there is a lack of vitamin D in the body of a pregnant woman, a child with a tendency to rickets may develop. Usually pregnant, and also during pregnancy planning, women are prescribed vitamin D. Its exact concentration, can only be determined by analysis. Also, the analysis is needed to control these indicators in dynamics, since the amount of vitamin should be adjusted to prevent either a lack or an overdose.
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How much analysis is done for vitamin D?
The analysis is done pretty quickly. Usually it takes 1 working day, less often - two. In many clinics, the results are reported immediately by telephone or e-mail.